Design, preparation, and characterization of silk fibroin/carboxymethyl cellulose wound dressing for skin tissue regeneration applications

Abstract Silk fibroin (SF) has been broadly applied in wound dressing fabrication because of its proper features for healing. In this work, we developed a carboxymethyl cellulose (CMC)/gelatin blend film with different concentrations glycerol, and modified the optimized an SF layer through electrospinning process. Tensile strength cell viability evaluation films demonstrated that glycerol content 3% could be suitable as substrate two‐layer dressing. The morphology electrospun nanofibers was obtained from scanning electron microscopy (SEM) Fourier transform infrared spectroscopy (FTIR). It concluded structural changes had occurred both layers after cross‐linking glutaraldehyde vapor. Further, it shown mechanical properties enhanced addition SF. Moreover, swelling ratio higher than those itself, due to hydrophilic property CMC/gelatin film. biocompatibility fibroblasts investigated by MTT assay, coating showed improvement proliferation cytocompatibility nature All results suggest prepared desirable candidate healing applications.